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Mutant microalgae produce wax esters for biofuel feedstock with improved cold flow
Date:
September 13, 2024
Source:
Osaka Metropolitan University
Summary:
Through genome editing using CRISPR/Cas9, researchers stably create euglena mutants that can produce wax esters with improved cold flow, making the esters suitable as feedstock for biofuels.
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News about biofuels sometimes mentions used cooking oil as a feedstock, but if these substances contain animal fat, they can solidify in colder temperatures. This happens because, chemically, the fatty acids of these and many other saturated fats have long carbon chains with single bonds. Enter the euglena. An Osaka Metropolitan University team has found a way to have one species of this microalgae produce wax esters with shorter carbon chains than usual.
Using CRISPR/Cas9 to edit the genome of Euglena gracilis, Dr. Masami Nakazawa and her team at the Graduate School of Agriculture’s Department of Applied Biochemistry produced stable mutants that created wax esters two carbons shorter than the wild-type species.
This improvement in the cold flow of the wax esters makes them more applicable as feedstock for biofuels. Among the factors favorable to using Euglena gracilis as a biofuel source are its ability to grow easily through photosynthesis and anaerobic production of wax esters.
“This achievement is expected to serve as a fundamental technology for replacing some petroleum-based production of wax esters with biological sources,” Dr. Nakazawa affirmed.
Story Source:
Materials provided by Osaka Metropolitan University . Note: Content may be edited for style and length.
Journal Reference:
Sakura Nagamine, Rikuto Oishi, Mitsuhiro Ueda, Tatsuji Sakamoto, Masami Nakazawa. Genome editing-based mutagenesis stably modifies composition of wax esters synthesized by Euglena gracilis under anaerobic conditions. Bioresource Technology, 2024; 410: 131255 DOI: 10.1016/j.biortech.2024.131255
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Osaka Metropolitan University. “CRISPR/Cas9 modifies euglena to create potential biofuel source.” ScienceDaily. ScienceDaily, 13 September 2024. .
Osaka Metropolitan University. (2024, September 13). CRISPR/Cas9 modifies euglena to create potential biofuel source. ScienceDaily. Retrieved September 16, 2024 from www.sciencedaily.com/releases/2024/09/240913105333.htm
Osaka Metropolitan University. “CRISPR/Cas9 modifies euglena to create potential biofuel source.” ScienceDaily. www.sciencedaily.com/releases/2024/09/240913105333.htm (accessed September 16, 2024).
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